||Dichroa febrifuga extract .............
||Coptis teeta extract ....................
||Qinghao leaf extract ...................
Dichroa febrifuga : Alkaloid, a, b and g-dichroine,
dichroidine and 4-quinazolone have been isolated from the root and
proved to be the active principles.
Coptis teeta : Berberine, coptine, coptisine,
jatrorhizine and palmatine have been isolated from the root and proved
to be the active principles.
Quinghao : Artemisinin was originally developed in
1972 in China from the plant known as Quinghao (Artemisia annua).
Artemisinin is the active ingredient in quinghao, a Chinese herbal tea
that have been used for 150 years to treat malaria.
The manner in which Plasmogine® kills off the
malarial parasite is a complex matter and several different mechanisms
are at work. The main actions seem to be:
Disruption of haemoglobin catabolism in the
Damage to the haem detoxification system of the
Generation of free radicals from the sesquiterpene
lactone which attack the membrane of the
Alkylation of intracellular proteins in the parasite
either by free radicals or by the haem -
The ability of Plasmogine® is to increase the cure
rate in malaria and to prevent the development of resistance in the
In vivo Mouse Model
Plasmodium berghei - mouse model system as described
by Peters (1970 ) was used to screen the effect of the drugs. Both
therapeutic and suppressive tests were done to determine the efficacy of
Both the test (suppressive and therapeutic) showed the effectiveness of
Plasmogine® in ddy mice infected with Plasmodium berghei. The effective
dosage was observed to be 100 mg/ kg/ day for 4 days.
In vitro sensitivity assay
In vitro sensitivity assay of Plasmogine® was carried
out with two isolates of Plasmodium falciparum in microtitre plate
containing RPMI1640 (LPLF) medium using the methods described by WHO.
The concentrations tested were 25, 50, 100, and 200 µg/ml. The desired
concentrations of drug were obtained by dissolving in the culture
The in vitro schizont inhibition was recorded after
36-hr. The in vitro schizont inhibition was marked at the concentration
of 50 µg/ml with the average inhibition rate of 95% (mean of three
experiments with two isolates of Plasmodium falciparum).
Herbal treatment of malaria.
1. 12 years and above :
Two caplets orally at once followed by one caplets 8
hourly (total 20 caplets).
2. Under 12 years :
20 mg / kg (body weight) at once followed by 10 mg /
kg (body weight) 8 hourly for 5 consecutive days.
No acute toxicity was recorded in mice receiving a
dosage of 1600 mg/kg /day (200 times of human dose) for 14 days.
Pregnancy and lactating mother.
10 caplets in one strip. 2 strips per box.